Determining the Potential Activity of Wnt Signaling During Zebrafish Oocyte Maturation
نویسندگان
چکیده
4 Introduction 4 A. Zebrafish as a Model Organism for Developmental Biology 4 B. Processes of Oocyte Maturation in Zebrafish 5 C. Wnt Signaling Pathways and Their Possible Role in Oocyte Maturation 11 D. Preliminary Findings and Objectives of This Research Project 15 Materials and Methods 16 A. Overview 16 B. Oocyte Harvesting and in vitro Maturation 17 C. RNA Extraction and cDNA Conversion 18 D. qPCR 18 E. Oocyte Fixation and Confocal Microscopy 19 F. Statistical Analysis 20 Results and Discussion 21 A. Initial Findings – Confocal Microscopy 21 B. qPCR Gene Expression Study 24 C. Conclusions and Future Directions 31
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